Bubbling bioreactor used for plant tissue culture

一种植物组织培养用鼓泡式生物反应器

Abstract

本发明公开了一种植物组织培养用鼓泡式生物反应器,包括由上下连接的球冠体结构和圆锥体结构构成的生物反应器主体;生物反应器主体侧壁设置有上视镜和下视镜,球冠体结构顶部连接有内表面安装灯镜的顶盖,顶盖上设置有接种口、补料口、排气冷却器接口和电极接口;圆锥体结构外部安装有控温夹套,圆锥体结构底部设置有连接空气进口的环形气体分布器,圆锥体结构底端连接有出料取样阀;生物反应器主体依次通过空气过滤器、空气滤罐与空气压缩机连接。本发明主体结构为近球形结构,在组培过程中克服了现有生物反应器容易产生气泡的缺点,从而避免了植物细胞受到气泡中的挥发性物质二氧化碳之类气体的影响,对植物细胞、组织和器官的剪切力较小。
The invention discloses a bubbling bioreactor used for plant tissue culture. The bioreactor comprises a bioreactor main body composed of an upper spherical cap structure and a lower cone structure in connection; upper sight glass and lower sight glass arranged on the side wall of the bioreactor main body, a top cover equipped with a lamp mirror and connected to the spherical cap top, an inoculation port, a feeding port, an exhaust cooler interface and an electrode interface installed on the top cover; a temperature control jacket mounted outside the cone structure, an annular gas distributor disposed at the bottom of the cone structure and connected to an air inlet, a discharge sampling valve in connection with the bottom end of the cone structure. The bioreactor main body is connected toan air compressor through an air filter and an air filtration pot in order. The main body of the invention is in a nearly spherical structure, and the disadvantage that bubbles are likely to be generated during tissue culture in present bioreactors can be overcome, so that the influence of volatile substances like carbon dioxide gas in the bubbles on plant cells can be avoided. Also the bioreactor of the invention has a small shearing force on plant cells, tissues and organs.

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Cited By (2)

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    CN-102757894-AOctober 31, 2012济南大学倒锥式微孔鼓泡液相深层发酵装置
    CN-103031251-AApril 10, 2013上海交通大学Multifunctional plant excised root culture bioreactor system