一株适应幼仓鼠肾传代细胞的新城疫病毒耐热弱毒株及其制备方法和应用

Newcastle disease virus heat-resistant attenuated strain adapted to baby hamster kidney passage cell and preparation method and application thereof

Abstract

本发明涉及一株适应幼仓鼠肾传代细胞的新城疫病毒耐热弱毒株及其制备方法和应用,其微生物保藏号是:CCTCC V201113;分类命名:新城疫病毒TS09-C株;保藏时间是:2011年5月9日;保藏单位:中国典型培养物保藏中心;保藏地址:中国.武汉.武汉大学,具有以下优点:1、已经充分适应BHK21细胞,其增殖滴度可达108.0TCID50/ml;2、具有较好的耐热特性,56度处理1小时HA效价无明显下降;3、具有弱毒特性,鸡胚平均致死时间大于90小时,脑内致病指数为0.00;4、以该毒株制备的新城疫耐热活疫苗(传代细胞源)具有耐热、保存期长、保护力好、可避免生产用鸡胚携带异源病毒等优点。
The invention relates to a newcastle disease virus heat-resistant attenuated strain adapted to baby hamster kidney passage cells and a preparation method and an application thereof, and the microbial preservation number is CCTCC No.V201113; the classification term is newcastle disease virus TS09-C strain; the preservation time is May 9th, 2011; the preservation unit is China center for type culture collection; the preservation address is Wuhan university, Wuhan, China; the strain has the following advantages: 1. the strain is fully adapted to BHK21 cells, and has a proliferation titer of up to 108.0TCID50/ml; 2. the strain has good heat resistance, and the HA titer does not decrease obviously after the strain is treated at 56 degree for 1 hour; 3. the strain has an attenuated characteristic, has chicken embryo average lethal time of more than 90 hours, and an intracerebral pathogenicity index of 0.00; 4. a newcastle disease heat-resistant live vaccine (passage cell source) prepared by the attenuated strain has the advantages of heat resistance, long storage life, good protective efficacy, prevention of chicken embryos for laying from carrying heterogenous virus, and the like.

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